Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.
Identifieur interne : 002869 ( Main/Exploration ); précédent : 002868; suivant : 002870Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.
Auteurs : Hui Wei [États-Unis] ; Melvin P. Tucker ; John O. Baker ; Michelle Harris ; Yonghua Luo ; Qi Xu ; Michael E. Himmel ; Shi-You DingSource :
- Biotechnology for biofuels [ 1754-6834 ] ; 2012.
Abstract
BACKGROUND
Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in biomass-to-biofuels conversion technology, which is the still-costly deconstruction of polymeric biomass carbohydrates to fermentable sugars.However, the complexity of both the structure of plant biomass and its counterpart microbial degradation communities makes it difficult to investigate the composting process.
RESULTS
In this study, a composter was set up with a mix of yellow poplar (Liriodendron tulipifera) wood-chips and mown lawn grass clippings (85:15 in dry-weight) and used as a model system. The microbial rDNA abundance data obtained from analyzing weekly-withdrawn composted samples suggested population-shifts from bacteria-dominated to fungus-dominated communities. Further analyses by an array of optical microscopic, transcriptional and enzyme-activity techniques yielded correlated results, suggesting that such population shifts occurred along with early removal of hemicellulose followed by attack on the consequently uncovered cellulose as the composting progressed.
CONCLUSION
The observed shifts in dominance by representative microbial groups, along with the observed different patterns in the gene expression and enzymatic activities between cellulases, hemicellulases, and ligninases during the composting process, provide new perspectives for biomass-derived biotechnology such as consolidated bioprocessing (CBP) and solid-state fermentation for the production of cellulolytic enzymes and biofuels.
DOI: 10.1186/1754-6834-5-20
PubMed: 22490508
PubMed Central: PMC3384452
Affiliations:
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Baker</name></author><author><name sortKey="Harris, Michelle" sort="Harris, Michelle" uniqKey="Harris M" first="Michelle" last="Harris">Michelle Harris</name></author><author><name sortKey="Luo, Yonghua" sort="Luo, Yonghua" uniqKey="Luo Y" first="Yonghua" last="Luo">Yonghua Luo</name></author><author><name sortKey="Xu, Qi" sort="Xu, Qi" uniqKey="Xu Q" first="Qi" last="Xu">Qi Xu</name></author><author><name sortKey="Himmel, Michael E" sort="Himmel, Michael E" uniqKey="Himmel M" first="Michael E" last="Himmel">Michael E. Himmel</name></author><author><name sortKey="Ding, Shi You" sort="Ding, Shi You" uniqKey="Ding S" first="Shi-You" last="Ding">Shi-You Ding</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22490508</idno><idno type="pmid">22490508</idno><idno type="doi">10.1186/1754-6834-5-20</idno><idno type="pmc">PMC3384452</idno><idno type="wicri:Area/Main/Corpus">002A81</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002A81</idno><idno type="wicri:Area/Main/Curation">002A81</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002A81</idno><idno type="wicri:Area/Main/Exploration">002A81</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.</title><author><name sortKey="Wei, Hui" sort="Wei, Hui" uniqKey="Wei H" first="Hui" last="Wei">Hui Wei</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. Hui.Wei@nrel.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Tucker, Melvin P" sort="Tucker, Melvin P" uniqKey="Tucker M" first="Melvin P" last="Tucker">Melvin P. Tucker</name></author><author><name sortKey="Baker, John O" sort="Baker, John O" uniqKey="Baker J" first="John O" last="Baker">John O. Baker</name></author><author><name sortKey="Harris, Michelle" sort="Harris, Michelle" uniqKey="Harris M" first="Michelle" last="Harris">Michelle Harris</name></author><author><name sortKey="Luo, Yonghua" sort="Luo, Yonghua" uniqKey="Luo Y" first="Yonghua" last="Luo">Yonghua Luo</name></author><author><name sortKey="Xu, Qi" sort="Xu, Qi" uniqKey="Xu Q" first="Qi" last="Xu">Qi Xu</name></author><author><name sortKey="Himmel, Michael E" sort="Himmel, Michael E" uniqKey="Himmel M" first="Michael E" last="Himmel">Michael E. Himmel</name></author><author><name sortKey="Ding, Shi You" sort="Ding, Shi You" uniqKey="Ding S" first="Shi-You" last="Ding">Shi-You Ding</name></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="eISSN">1754-6834</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in biomass-to-biofuels conversion technology, which is the still-costly deconstruction of polymeric biomass carbohydrates to fermentable sugars.However, the complexity of both the structure of plant biomass and its counterpart microbial degradation communities makes it difficult to investigate the composting process.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In this study, a composter was set up with a mix of yellow poplar (Liriodendron tulipifera) wood-chips and mown lawn grass clippings (85:15 in dry-weight) and used as a model system. The microbial rDNA abundance data obtained from analyzing weekly-withdrawn composted samples suggested population-shifts from bacteria-dominated to fungus-dominated communities. Further analyses by an array of optical microscopic, transcriptional and enzyme-activity techniques yielded correlated results, suggesting that such population shifts occurred along with early removal of hemicellulose followed by attack on the consequently uncovered cellulose as the composting progressed.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The observed shifts in dominance by representative microbial groups, along with the observed different patterns in the gene expression and enzymatic activities between cellulases, hemicellulases, and ligninases during the composting process, provide new perspectives for biomass-derived biotechnology such as consolidated bioprocessing (CBP) and solid-state fermentation for the production of cellulolytic enzymes and biofuels.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">22490508</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1754-6834</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Apr</Month><Day>10</Day></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.</ArticleTitle><Pagination><MedlinePgn>20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1754-6834-5-20</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in biomass-to-biofuels conversion technology, which is the still-costly deconstruction of polymeric biomass carbohydrates to fermentable sugars.However, the complexity of both the structure of plant biomass and its counterpart microbial degradation communities makes it difficult to investigate the composting process.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In this study, a composter was set up with a mix of yellow poplar (Liriodendron tulipifera) wood-chips and mown lawn grass clippings (85:15 in dry-weight) and used as a model system. The microbial rDNA abundance data obtained from analyzing weekly-withdrawn composted samples suggested population-shifts from bacteria-dominated to fungus-dominated communities. Further analyses by an array of optical microscopic, transcriptional and enzyme-activity techniques yielded correlated results, suggesting that such population shifts occurred along with early removal of hemicellulose followed by attack on the consequently uncovered cellulose as the composting progressed.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The observed shifts in dominance by representative microbial groups, along with the observed different patterns in the gene expression and enzymatic activities between cellulases, hemicellulases, and ligninases during the composting process, provide new perspectives for biomass-derived biotechnology such as consolidated bioprocessing (CBP) and solid-state fermentation for the production of cellulolytic enzymes and biofuels.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. 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Baker</name><name sortKey="Ding, Shi You" sort="Ding, Shi You" uniqKey="Ding S" first="Shi-You" last="Ding">Shi-You Ding</name><name sortKey="Harris, Michelle" sort="Harris, Michelle" uniqKey="Harris M" first="Michelle" last="Harris">Michelle Harris</name><name sortKey="Himmel, Michael E" sort="Himmel, Michael E" uniqKey="Himmel M" first="Michael E" last="Himmel">Michael E. Himmel</name><name sortKey="Luo, Yonghua" sort="Luo, Yonghua" uniqKey="Luo Y" first="Yonghua" last="Luo">Yonghua Luo</name><name sortKey="Tucker, Melvin P" sort="Tucker, Melvin P" uniqKey="Tucker M" first="Melvin P" last="Tucker">Melvin P. Tucker</name><name sortKey="Xu, Qi" sort="Xu, Qi" uniqKey="Xu Q" first="Qi" last="Xu">Qi Xu</name></noCountry><country name="États-Unis"><region name="Colorado"><name sortKey="Wei, Hui" sort="Wei, Hui" uniqKey="Wei H" first="Hui" last="Wei">Hui Wei</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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